FAQ

SPL Product Raw material Chemical Resistance Chart


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What is the difference between the SPL coated products (SPLCoat™) and the surface treated cell cult

Cell culture products are surface treated to modify electrostatic surface of polystyrene for better cell adhesion.
Therefore, anchorage-dependent cells tend to grow stably due to its hydrophilic nature. On the other hand,
SPLCoat™ products are coated with biological substrates for better cell adhesion. For more information, please refer to the production descriptions.

Can non-sterile cell culture products cause contamination?

Generally, cell culture products are sterilized before use. However, cell adhesion may significantly be affected by sterilization.
We provide non-sterile products in case of some cells being negatively affected by this process. Non-sterile products are produced
through automated manufacturing process in high pressing temperature, and packaged right away. Therefore, the products are less
likely provided in contaminated conditions, but there is no guarantee for potential contamination during the experimental procedure.  

Can I use cell culture plate for ELISA (Enzyme-Linked Immunosorbent Assay)?

The surface treatment methods for cell culture plate and immunoplate are different, and thus your results,
 such as experiments using protein coating, may be inaccurate. We recommend using appropriate plates for each purpose for the best outcome.

What is the difference between White and Black plates?

Researchers are recommended to choose products that best suit their experimental purposes.
Black plate is used for fluorescence assay, minimizing well-to-well interaction and light scattering.
White plate is used for luminescence assay for maximal light reflection with minimal well-to-well interaction.
 Misapplication may cause inaccurate experimental results. We recommend using adequate plate for the best outcome.

There are so many types of SPLInsert™. How should I choose the right product for my experiment?

SPLInsert™ products are provided with various forms, sizes, materials, and pore sizes for different experiment purposes.
The inserts are designed as either hanging on the plate well or standing on the bottom. Both forms are provided with 6well plate and 24well plate.
 6 inserts will be provided in one 6well plate, while 12 inserts will be provided in one 24well plate. SPLInsert™ is composed of inserts and multiwall plate.
 
PC membrane is stain-free and has low background interference characteristic, while PET membrane has higher chemical resistance with
better protein adhesion. If this is your first time using SPLInsert™, we recommend using PC membrane. In addition, PET membrane is recommended
if you are looking for optically clear inserts.

0.4 ㎛ pore-sized membrane is ideal for drug transport study without cell migration. Since 3.0 and 8.0 ㎛ pore-sized membranes are large enough
 pathway for cells, they are ideal for cell invasion, migration, chemotaxis assay, motility study, etc. You can choose adequate product based on
 your cell size and migration ability.


If you are still not sure of what to choose for your experiment, please refer to peer-reviewed publications citing insert (of other brands) used in experiments similar to yours.

What is the difference between DLux and FLux?

DLux and FLux surfaces are engineered plastic bottoms for better cell adhesion, compared to regular glass bottoms.
The products are surface treated, and have low auto-flourescence. DLux has identical thickness to out slide glass (approx. 1 mm),
with similar performance to our glass bottoms. FLux has the thickness of cover slip (188 ㎛), optimized for live cell imaging. 

Are SPLScar™ Scratcher and Block reusable?

SPLScar™ products are used for wound healing assays, creating 500 ㎛ cell free gaps on cell cultured surfaces.


SPLScar™ Scratcher is composed of a tip (500 ㎛ thin) that may deform unevenly when autoclaved for reuse.
Such tip may not be ideal for creating uniform scratches for wound healing assays.
In the case of SPLScarTM Block, the initial adhesive property may be lost after cleansing/autoclaving for reuse.