FAQ

Can I use cell culture plate for ELISA (Enzyme-Linked Immunosorbent Assay)?

The surface treatment methods for cell culture plate and immunoplate are different, and thus your results,
 such as experiments using protein coating, may be inaccurate. We recommend using appropriate plates for each purpose for the best outcome.

What is the difference between White and Black plates?

Researchers are recommended to choose products that best suit their experimental purposes.
Black plate is used for fluorescence assay, minimizing well-to-well interaction and light scattering.
White plate is used for luminescence assay for maximal light reflection with minimal well-to-well interaction.
 Misapplication may cause inaccurate experimental results. We recommend using adequate plate for the best outcome.

There are so many types of SPLInsert™. How should I choose the right product for my experiment?

SPLInsert™ products are provided with various forms, sizes, materials, and pore sizes for different experiment purposes.
The inserts are designed as either hanging on the plate well or standing on the bottom. Both forms are provided with 6well plate and 24well plate.
 6 inserts will be provided in one 6well plate, while 12 inserts will be provided in one 24well plate. SPLInsert™ is composed of inserts and multiwall plate.
 
PC membrane is stain-free and has low background interference characteristic, while PET membrane has higher chemical resistance with
better protein adhesion. If this is your first time using SPLInsert™, we recommend using PC membrane. In addition, PET membrane is recommended
if you are looking for optically clear inserts.

0.4 ㎛ pore-sized membrane is ideal for drug transport study without cell migration. Since 3.0 and 8.0 ㎛ pore-sized membranes are large enough
 pathway for cells, they are ideal for cell invasion, migration, chemotaxis assay, motility study, etc. You can choose adequate product based on
 your cell size and migration ability.


If you are still not sure of what to choose for your experiment, please refer to peer-reviewed publications citing insert (of other brands) used in experiments similar to yours.

What is the difference between DLux and FLux?

DLux and FLux surfaces are engineered plastic bottoms for better cell adhesion, compared to regular glass bottoms.
The products are surface treated, and have low auto-flourescence. DLux has identical thickness to out slide glass (approx. 1 mm),
with similar performance to our glass bottoms. FLux has the thickness of cover slip (188 ㎛), optimized for live cell imaging. 

Are SPLScar™ Scratcher and Block reusable?

SPLScar™ products are used for wound healing assays, creating 500 ㎛ cell free gaps on cell cultured surfaces.


SPLScar™ Scratcher is composed of a tip (500 ㎛ thin) that may deform unevenly when autoclaved for reuse.
Such tip may not be ideal for creating uniform scratches for wound healing assays.
In the case of SPLScarTM Block, the initial adhesive property may be lost after cleansing/autoclaving for reuse.

What is special about SPLPermea™ Bags?

SPLPermea™ Bags are a cell culture device, designed to provide maximal oxygen and carbon dioxide exchange,
minimizing the acidification of culture medium. The bags are made of engineered membrane for better gas exchange,
compared to other existing cell culture devices (e.g. cell culture flask). With minimal medium evaporation and acidification,
both adhesion and suspension cells will grow effectively in large scale, suitable for industrial use. In addition, SPLPermea™ Bags are
provided with its uniquely designed rack for easier handling, unlike culture bags of other brands.

How are Immunoplates categorized?

SPL Immunoplates can be categorized based on hydrophilic/hydrophobic property of the surface.
The surfaces are arranged in order of hydrophilic property, starting from Multibinding, Maxibinding, Medibinding, and Unibinding.
You can choose the adequate surface based on hydrophilic property that suits your coating material the most.
If you are not sure which surface is suitable for your coating material, or if this is your first time trying our immunoplate,
we recommend using Maxibinding surface. If Maxibinding is not appropriate, we would like you to try other types of surface to
 find the most suitable one for your experiment.

What is special about 1.5ml Strip Tube?

1.5 ml Strip Tubes are composed of 6 microcentrifuge tubes connected physically in a row,
ideal for experiments requiring large sample number. The tubes in strip can be cut for individual use,
and applicable to centrifugation. It is worth noting that the strip may not be applicable to some rotors for centrifugation
(applicable to most 24-, 30- hole rotors, and some 18-hole rotor).
We recommend you to confirm rotor applicability before of your actual experiments.